Depression threads

**AoE** · 2012-05-30 04:59

They should be a bannable offense.

Circlest of jerk topics and poor trolling.

**Brill Weave** · 2012-05-30 06:16

Some of them can be fun to watch though. Most of them are just sad.

**Linliel** · 2012-05-30 06:21

AoE, what are you doing indoors? Get out in the sunshine while it's here!

**Brill Weave** · 2012-05-30 07:42

If he gets melanoma you will have blood on your hands woman.

**Linliel** · 2012-05-30 07:47

He's not going to get a melanoma from the British sun!

**Brill Weave** · 2012-05-30 07:54

You guys don't have the sun over there. Who are you kidding. All you have is rain, crooked teeth, and Neville Chamberlain statues.

**Linliel** · 2012-05-30 09:26

Originally Posted by Brill Weave

You guys don't have the sun over there. Who are you kidding. All you have is rain, crooked teeth, and Neville Chamberlain statues.

Tell that to 2 weeks of sun and warm temps

**Qalbert** · 2012-05-30 09:40

is that the running total for the 2000s

**Linliel** · 2012-05-30 09:57

oh ho ho ho

**Charismatic** · 2012-05-30 13:50

Originally Posted by Brill Weave

Most of them are just sad.

Well...

**Chun Liroy** · 2012-05-30 14:07

FUCK IM DEPRESSED WHY ISNT THIS LAB SHIT WORKING IT SHOULD BE WORKING WHY DO I ALWAYS SCREW UP PCR PURIFICATION

**Chun Liroy** · 2012-05-30 14:07

amidoinitrite

**Shiroikage** · 2012-05-30 14:12

Originally Posted by Chun Liroy

FUCK IM DEPRESSED WHY ISNT THIS LAB SHIT WORKING IT SHOULD BE WORKING WHY DO I ALWAYS SCREW UP PCR PURIFICATION

eventually this will become unironic

**Chun Liroy** · 2012-05-30 14:23

Why is it ironic

**Chun Liroy** · 2012-05-30 14:23

Because I should be screwing up PCR and not the easy shit but I'm doing it vice versa?

**Shiroikage** · 2012-05-30 14:24

no, i mean it will actually be a real statement and not a joke statement

**Chun Liroy** · 2012-05-30 14:28

I wish I was joking. >100 ug in brightness after pcr before purification,

Abso-fucking-lutely nothing after purification

**Shiroikage** · 2012-05-30 14:31

Originally Posted by Chun Liroy

I wish I was joking. >100 ug in brightness after pcr before purification,

Abso-fucking-lutely nothing after purification

wait, you are losing it in purification, not extraction?
damn, man, that seriously blows

**Shiroikage** · 2012-05-30 14:31

assuming you're using some sort of spin column?
can't say i ever messed up a purification, extraction (hoo boy) that's a different story

**Chun Liroy** · 2012-05-30 14:33

Yes, spin column. I just don't get it either, have no idea what I'm doing wrong.

Buffer PB x5 > 750ul PE > dry spin > 30ul EB

Thread: Depression threads