I had to give a speech at my brother's wedding last year, and for like an entire year before it happened everyone was joking and ribbing me about how I had to give the speech and all that junk and how they were lookin forward to it, because I'm usually the quiet one in the family. In the end, I didn't really treat it like a big deal, and I didn't even start working on it until two nights before the wedding. I printed it out the day off (though I'll have to echo the whole "use cards" bit, paper sucks) and when it came time to it I basically just winged most of it, using what I wrote as nothing more then a guide.
I personally didn't think it was anything special, but apparently everyone else loved it. I wasn't smooth or anything, fumbled with the notes and probably ran on too long. But it was full of (clean) humorous stories about growing up with my bro and generally saying nice things about both of them in a funny way.
Best advice I can give about the actual speech:
Don't make it too long
Try and keep it entertaining, open up with a few jokes, specifically about how it was like living with your bro.
Stories from your childhood, preferably ones even your brother may have forgotten, are always good to use.
Say something nice about the wife, maybe something she did for you that you'll always appriciate yadda yadda, and welcome her to the family and all that.
And try and top it off with some sappy stuff about lovin both of them and being glad to know them and then the toast.
Basic advice about public speaking:
Relax. Most of the people there are family and won't care how you do. Anyone uptight enough to be bothered by how you talk isn't worth caring about in the end.
Don't be drunk, wait till after the speech to get hammered.
Try to make eye contact with people, on both sides of the room. Don't look down and read word for word, look up, look around, smile and try and act like you're speaking directly to someone.
Use your hands, emote, emphasize stuff, just don't stand there stiff as a board.
Use cards. Paper sucks. It's loud when it wrinkles, annoying to flip through, and it's easy to lose your place in big blocks of text.
And most important of all, relax. I know I said it already, but that's because it's doubley important. It's a wedding, a time to celebrate family, friends and have fun. So do just that, and don't sweat the small stuff. All the stress of a wedding should happen before the wedding. Everything else is when the party happens.
Just my two cents.
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